The offer of cash to help build a new London centre was initially accepted, but after the performance last Friday, Maggie's Centres received a phone call from the hardline Christian organisation.
Rebecca Rendle, fundraising director for Maggie's Centres, explained: "We have no objections to Jerry Springer - the Opera, but Christian Voice said if we accepted the donation we would offend tens of thousands of its members.
"We recognise that Christian Voice used this to publicise their activities. However, everything we do has to be guided by the people we provide a service to."
The national director of Christian Voice admitted that it had contacted the charity. Stephen Green said: "Many people with terminal cancer draw comfort from their Christian faith - to know that money was coming from a production like this just wouldn't be right."