Youth Action Network will close in September because of a lack of funds.
The Birmingham-based charity, which has about 100 youth organisations as members and employs five staff, received £158,000 from the Office for Civil Society's strategic partners programme in 2010/11.
But the charity's bid for funding from the OCS's new strategic partners transition fund for the next three years was rejected.
Davina Goodchild, its chief executive, said the organisation would have probably carried on had it been given more funding by the OCS.
YAN, founded in 1996 as the National Federation of Youth Action Agencies, will transfer its assets to the National Council for Voluntary Youth Services.
"We thought it was more sensible to close and find an elegant exit rather than limp on hoping for something to come up," said Goodchild.
The Council of Ethnic Minority Voluntary Sector Organisations has warned it could also close after the end of a number of contracts and the loss of funding from the strategic partners programme.
Hashmukh Pankhania, chief executive of Cemvo, said it had reduced the number of full-time staff from 10 to three, and the number of project staff had gone from 12 to three.
He said the organisation had only one and a half contracts remaining, worth a total of £117,000. A number of its other contracts had come to an end recently, he said, including one for Essex County Council, which was worth £1.8m to the organisation over the past three years.
Cemvo received £275,953 from the strategic partners programme in 2010/11, but its application for further funding was turned down.
Asked whether he thought the organisation would have to close, Pankhania said it was a possibility but he was trying to remain optimistic about Cemvo being subcontracted for work from the Welfare to Work programme.
"But it's possible that if we don't, we'll have to close or amalgamate with another organisation," he said.
"The future of the organisation is precarious. Our survival depends on the next 12 months. We have to look at different ways of generating income."
Pankhania said Cemvo was closing its offices in Chelmsford, Manchester and Bristol, so only its London office would remain open.