Almost 1,600 Scope staff have completed their transfer to a private care company as part of the disability charity's new strategy.
Third Sector revealed in December that Scope had agreed to sell its regulated and day services at 51 locations in England and Wales to the Windsor-based Salutem Healthcare.
The sale is part of plans to focus on advocacy, information and support for disabled people and no longer provide care services.
Mark Atkinson, chief executive of Scope, revealed in a series of tweets yesterday that the deal had been finalised.
"Yesterday evening we transferred our portfolio of 55 regulated and day services to another provider," he said.
"Thank you to the 1,598 front-line employees who continue their important work with a new employer."
Atkinson said it had been a "long and complex journey", but the move meant Scope could invest its energy "in the areas that reach the most people and achieve the biggest impact in tackling equality".
He added: "We are determined that Scope will be an organisation relevant to the lives of millions of disabled people by being there at the moments that matter.
"That required us to refocus, make difficult choices and move away from being a provider of regulated and day services.
"I am proud that we have taken the big decisions, which allow us to be a truly mission-led organisation. We are at our best when at our boldest."
The staff in question joined Ambito Care & Education, a division of Salutem, under the Transfer of Undertakings (Protection of Employment) Regulations, known as Tupe.
Scope's remaining 868 staff are due to move to new offices at Here East, part of the London Olympic Park, on 15 May.
A Scope spokesman said the charity would reveal the sum paid for the homes in its next annual report.
Based on its recent filing history with the Charity Commission, the charity is likely to file its accounts for the year to the end of March 2018 in December.