It will be the first time the three princes have ever given an interview together.
“We enjoyed interviewing the Prince of Wales four years ago and we’re thrilled to have the opportunity to interview him again,” said Ant and Dec.
Prince’s Trust 30th Live, to be held next May, will also feature a concert at the Tower of London.
Tickets for the concert will be available through a text ballot. TV viewers will be asked to pledge money to the charity.
The trust will hold the UK finals of its Celebrate Success Awards in February at the Theatre Royal in London. This annual event recognises achievements by young people who have succeeded against the odds.