A Barclays branch is objecting to processing charity donors' cheques itself on the grounds that it is too much work.
Pamela Goldberg, chief executive of Breast Cancer Campaign, revealed her concerns about the issue on her blog last week.
She told Third Sector that a colleague was asked to stop taking cheques into one branch of Barclays because of the work involved in processing them. The employee was told to use the bank's service that sends cheques to a central sorting office, but Goldberg said this had proved to be "a nightmare" in the past when the bank lost a bag of cheques.
"Processing cheques must be a tedious job, but that's not my problem," she said.
Lindsay Boswell, chief executive of the Institute of Fundraising, said it was a continuing issue for charities.
He said it was inevitable that cheques would eventually disappear, but while banks were still issuing cheque books they should be willing to carry out the processing involved.
A Barclays spokesman said: "There are a number of options to help businesses and charities process cheques as quickly and conveniently as possible. If charities want to continue queuing at the till, they can."
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