Asthma UK could shed up to a third of its staff in a bid to cut costs.
The charity is proposing to reduce staffing levels drastically as part of a wider review of how it can save money without taking funds away from essential work.
Up to 32 out of 109 jobs could be lost if all the proposed cuts go ahead. Senior management and support roles will be most affected.
Twenty posts have been selected for compulsory and voluntary redundancies. Seven vacancies will not be filled, and a further five empty posts are under review. More jobs might remain unfilled as people leave if their roles are not deemed to be critical.
The charity blamed the losses on a drop in income, which fell from £11.04m in 2008 to £8.95m in 2009. To balance this, it aims to cut its non-charitable spending by £800,000 this year. Three-quarters of this will come from its payroll budget.
Neil Churchill, chief executive of Asthma UK, said: "In line with many charities across the sector, we are facing growing demand but greater pressure on revenues.
"Since the recession started we have avoided making redundancies, but are now having to make changes to ensure the future efficiency and effectiveness of the organisation."
Asthma UK implemented a partial recruitment freeze last year as a result of the recession.
A spokeswoman said the charity had created some new roles and hoped to redeploy redundant staff where appropriate.