The Co-operative Bank has extended the range of its Customers Who Care scheme, which has raised more than £2 million for charities since 1994.
As part of its ethical policy, the bank donates £1.25 for every £100 spend on customers' Visa credit and debit cards to an annual charity campaign or to nominated charities.
This has now been extended to business Visa debit and business Visa charge cards.
Bryce Glover, corporate and business banking director at the Co-operative Bank, said: "It makes good business sense for ourselves and our customers to become involved in the scheme.
"A recent review of our ethical policy highlighted that our business customers are just as ethically minded as our personal customers."
This year's campaign has supported Landmine Action in its bid to stop the use of cluster bombs.