The environmental charity Groundwork Leicester and Leicestershire has gone into voluntary liquidation with the immediate loss of 26 jobs.
The charity, which provided a range of services including training about recycling, closed on Friday.
It registered with the Charity Commission in 1990 and accounts submitted to the regulator show that it had an income of £1.24m and outgoings totalling £1.25m in 2011/12.
The accountancy firm BDO, which is handling the liquidation process, said that 26 members of staff were made redundant when the charity closed last week. A further five staff members will continue to work for the charity as it winds down, but will lose their jobs at the end of the process.
A spokeswoman for BDO said: "Groundwork Leicester and Leicestershire, a local environmental organisation, ceased to trade on 3 May 2013.
"Creditors are being advised of the circumstances, and a meeting of the creditors is being arranged for later this month."
The spokeswoman declined to provide details on why the charity had gone into liquidation.