The move will offer members of Acre, which is the national body for 38 rural community councils across England, free access to the benefits of NCVO membership.
The partnership was announced yesterday at the parliamentary reception of the All-Party Parliamentary Group on Charities and Volunteering, organised by the NCVO.
The NCVO said the move would bring an "important rural and local perspective" to its national policy work.
Acre, which was formed in 1987 by the NCVO, will remain independent under the new partnership, which is not a merger.
Sir Stuart Etherington, chief executive of the NCVO, said: "This is an important partnership, not only strengthening our understanding of issues affecting rural communities, but also providing a greater level of support and advice to vital rural networks."
Richard Quallington, executive director of Acre, said: "Since becoming independent from NCVO 31 years ago, Acre has had a strong track record of speaking up for rural communities on the national stage. We are looking forward to working with the NCVO to further strengthen and amplify this rural voice."