The RSPB is waiting to hear today whether its single, which consists entirely of birdsong, has reached the top 10.
Let Nature Sing, which was released a week ago, hit number 11 in the midweek charts this week.
Now the charity is hoping to feature alongside the likes of Stormzy and Taylor Swift on the official singles chart top 10, which is based on sales of downloads, CDs and video streams.
It is the first single featuring only birdsong to make the charts.
Rebecca Munro, director of fundraising and communications at the RSPB, said it produced the single to raise awareness of Britain's declining bird numbers.
"It's not a fundraising exercise at all for us," said Munro. "Even if it gets into the top 10 it would only generate a small amount of funds."
She said the charity was good at talking to politicians and policy-makers, but less accomplished at engaging with members of the public, and decided music was a great way to achieve this.
It hired the creative agency Glimpse to produce the two-minute 28-second video, which features 25 different types of birdsong.
It ends with the message that since 1966 the UK has lost more than 40 million birds and the RSPB is working to save those that remain.
The single was released to coincide with International Dawn Chorus Day on Sunday.
Martin Harper, director of conservation at the RSPB, said in a statement: "The response to Let Nature Sing sends a powerful message that, yes, nature is amazing, but it is also in trouble.
"The good news is that it is not too late, we know what needs to be done and together we can take action to restore it for us and for future generations."