Sally Burton, chief executive of the Shaw Trust, has left the charity with immediate effect.
A spokesman for the trust, which provides employment opportunities for disabled people, said Burton’s departure was a "mutual decision".
He declined to confirm the reason for her departure, which was announced to staff on Monday. He also said he had "no details" of whether she had received a pay-off and said he could not confirm whether she had a job to go to.John Briffitt, the trust's chair, said: "We are very grateful for her commitment to our mission to help people with disability and those at disadvantage find employment and live more independent lives."
Burton became chief executive of the Shaw Trust in November 2009. Prior to joining the trust, she had spent 25 years in the public sector and was the first chief executive of Solihull NHS Care Trust.
Her predecessor at the Shaw Trust, the long-serving Ian Charlesworth, was dismissed in August 2008 after a row among management and trustees over plans to expand into Australia.
The organisation will be jointly run in the short-term by Steve Offord, the trust’s executive director of HR, and Jerome Walls, the executive director of finance.
A Shaw Trust statement said the charity is "acting to appoint a replacement" for Burton.