The suicide-prevention charity abandoned the appointment of Hughes last week after newspaper reports alleged that he displayed bullying behaviour during his time at the Alzheimer’s Society.
Samaritans said in a statement that it “cannot proceed with the appointment” of Hughes, who was due to take up the post in May.
But the charity said the decision was not based on the allegations, which it said it was not in a position to judge.
A spokeswoman for the charity told Third Sector there had been no financial settlement with Hughes after the cancellation of his appointment.
Hughes stepped down early from his role at the Alzheimer’s Society this week, having originally been due to leave in April.
His replacement, Kate Lee, formerly of the young people’s cancer charity Clic Sargent, brought forward her appointment from April to this week as a result of Hughes’s early departure.
The Charity Commission has also reopened a 2018 complaint about the handling of staff grievances at the Alzheimer’s Society, and the regulator has apologised for failing to follow up properly at the time.