Save the Children will open concessions in branches of the department store Beales in Worthing, West Sussex, and Bedford this week.
The concessions will be part of the Hallett Retail umbrella concessions, called Fuse. Some of the charity brands on sale in Fuse have agreed to donate stock to the Save the Children concessions.
Jayne Cartwright, head of retail partnerships at Save the Children, said she hoped the concessions would prove charity retail was relevant to British shoppers.
"I wanted to look outside the box," she said. "I think it's important that charities keep looking at new opportunities to raise money."
Save the Children will not incur any costs, with Beales and Hallett Retail covering all overheads.
Wendy Hallett, the founder of Hallett Retail, said the long-term aim was to raise £1m for Save the Children by eventually opening more concessions.
She said the initial focus would be to keep the first two concessions fully stocked and ensuring that they worked well.
The concession at the Worthing store was due to be launched today, with the Bedford concession opening on 6 October.