The charity is the official charity partner of the Independent Schools Show, which takes place in London at the end of October.
The show offers parents the chance to talk to representatives from more than 120 fee-paying schools from across mainland Britain.
Duncan Batty, head of fundraising for children, young people and volunteers at Barnardo's, said the charity did not have a problem associating itself with independent schools.
"Barnardo's has not campaigned about independent education at all, so it is not something we are uncomfortable with," he said.
The charity will raise money by taking a proportion of funds generated by ticket sales, as well as from donations to support a Barnardo's project for children excluded from school.
It also hopes to use the show to establish contacts with wealthy parents and the schools.