Two members of the Shaw Trust's executive team have left the charity, which is the largest voluntary sector provider of employment services for disabled people.
Catherine A'Bear, chief officer for corporate affairs, and Karin Pappenheim, director for work and independence, both left the organisation on 23 December.
A spokeswoman for the Shaw Trust said A'Bear's departure was mutually agreed.
"All parties decided it was the right time for Catherine to move on and look at new challenges," she said. "It's no secret that we are looking at the organisational structure, particularly at leadership level."
Pappenheim joined the Shaw Trust in May 2009 after a merger with the charity Employment Opportunities, of which she was chief executive. The trust spokeswoman said Pappenheim's role was an interim position to oversee the integration of the two organisations and that it had "come to a natural end".
The departures come after former civil servant Sally Burton took over at the trust in November.
"When a new chief executive comes on board there are bound to be changes," said the spokeswoman.